Hotspot

trnC-GCA-petN

LSC · 9414-10246 · 833 bp

Back to Blumea

Pi 0.0184
MarkerSeek score 63.10
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
833
variable_sites
41
pi
0.0184
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TGTATATCGTTTTGGCGGCA AGCCCAAGCGAGACTTACTA 45.0 50.0 58.0 58.2 928-949 bp 1.0 83.8
trnC-GCA-petN_p2 TTTCCCCAGTTCAAATCCGG AGCCCAAGCGAGACTTACTA 50.0 50.0 58.1 58.2 873-894 bp 1.0 83.6
trnC-GCA-petN_p3 GGGGGACTGCAAATCCTTTT AGCCCAAGCGAGACTTACTA 50.0 50.0 58.4 58.2 891-912 bp 1.0 81.3
trnC-GCA-petN_p4 TGTATATCGTTTTGGCGGCA GCCCAAGCGAGACTTACTAT 45.0 50.0 58.0 57.1 927-948 bp 1.0 77.8
trnC-GCA-petN_p5 TGTATATCGTTTTGGCGGCA ATTAAAGCAGCCCAAGCGAG 45.0 50.0 58.0 58.9 936-957 bp 1.0 77.7