Hotspot

trnH-GUG-psbA

LSC · 77-464 · 388 bp

Back to Blumea

Pi 0.0325
MarkerSeek score 69.50
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
388
variable_sites
33
pi
0.0325
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.91
markerseek_score
69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG CCGTGCTAACCTTGGTATGG 55.0 55.0 58.1 58.3 479-514 bp 1.0 85.6
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 493-528 bp 1.0 85.4
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 492-527 bp 1.0 85.4
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 494-529 bp 1.0 85.4
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG ACCGTGCTAACCTTGGTATG 55.0 50.0 58.1 57.6 480-515 bp 1.0 85.1