Hotspot

rpl22

LSC · 83473-84072 · 600 bp

Pi 0.0233

MarkerSeek score 58.30

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 600
variable_sites: 14
pi: 0.0233
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	CAAGTCGGAAACCAAGTGGA	TGGGTAGAGCCGGATCTAAG	50.0	55.0	58.0	58.0	1272-1273 bp	1.0	80.9
rpl22_p2	ATCGATACGGCCTGCAATTT	TGGGTAGAGCCGGATCTAAG	45.0	55.0	58.0	58.0	1742-1743 bp	1.0	80.8
rpl22_p3	TTTTCCATCGATACGGCCTG	TGGGTAGAGCCGGATCTAAG	50.0	55.0	58.1	58.0	1748-1749 bp	1.0	80.7
rpl22_p4	AGGGTGCCCATAAGGTTTTG	TGGGTAGAGCCGGATCTAAG	50.0	55.0	58.1	58.0	1607-1608 bp	1.0	80.6
rpl22_p5	ATACGGCCTGCAATTTGGAT	TGGGTAGAGCCGGATCTAAG	45.0	55.0	57.9	58.0	1738-1739 bp	1.0	80.5