Hotspot

ndhF

SSC · 109431-111665 · 2235 bp

Pi 0.0125

MarkerSeek score 58.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2235
variable_sites: 28
pi: 0.0125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGTTCTAGACAAAGGACT	CAAGAGCCGGTGAATCGTAA	40.0	50.0	53.2	58.0	2355 bp	1.0	42.7
ndhF_p2	ACAGTTCTAGACAAAGGACTT	CAAGAGCCGGTGAATCGTAA	38.1	50.0	54.1	58.0	2355 bp	1.0	42.0
ndhF_p3	ACAGTTCTAGACAAAGGACTTT	CAAGAGCCGGTGAATCGTAA	36.4	50.0	55.0	58.0	2355 bp	1.0	41.0
ndhF_p4	ACAGTTCTAGACAAAGGACTTTT	CAAGAGCCGGTGAATCGTAA	34.8	50.0	55.7	58.0	2355 bp	1.0	41.0
ndhF_p5	ACAGTTCTAGACAAAGGACT	AGCCGGTGAATCGTAAATCA	40.0	45.0	53.2	57.3	2351 bp	1.0	41.0