Hotspot

ndhA

SSC · 119291-121497 · 2207 bp

Pi 0.0114

MarkerSeek score 49.90

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2207
variable_sites: 25
pi: 0.0114
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACGAAAACGCTCCGATGTTA	CCGGAATACAATGGGACCTG	45.0	55.0	57.9	58.0	2965-2984 bp	1.0	80.6
ndhA_p2	ACGAAAACGCTCCGATGTTA	GAATACAATGGGACCTGCGT	45.0	50.0	57.9	58.0	2962-2981 bp	1.0	80.6
ndhA_p3	ACGAAAACGCTCCGATGTTA	TGGGAAGTCCAATGGCAAAA	45.0	45.0	57.9	57.9	2906-2925 bp	1.0	80.2
ndhA_p4	ACGAAAACGCTCCGATGTTA	GGGACCTGCGTAAAGTTGAT	45.0	50.0	57.9	57.9	2953-2972 bp	1.0	80.0
ndhA_p5	ACGAAAACGCTCCGATGTTA	TCCGGAATACAATGGGACCT	45.0	50.0	57.9	58.1	2966-2985 bp	1.0	80.0