Hotspot

ndhI-ndhA

SSC · 119180-119290 · 111 bp

Back to Blitum

Pi 0.045
MarkerSeek score 71.00
Primer available yes
Label ndhI-ndhA

Feature Metadata

label_name
ndhI-ndhA
feature_type
igs
region
SSC
length_bp
111
variable_sites
5
pi
0.045
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhI-ndhA_p1 TTGACCAATGTACCTTGCCG ACCTAGACTGAGAATGGACCA 50.0 47.6 58.5 57.5 292-293 bp 1.0 65.8
ndhI-ndhA_p2 CGCGTATTGTTTGTTGACCA ACCTAGACTGAGAATGGACCA 45.0 47.6 57.3 57.5 272-273 bp 1.0 64.3
ndhI-ndhA_p3 GCGCGTATTGTTTGTTGACC ACCTAGACTGAGAATGGACCA 50.0 47.6 58.7 57.5 273-274 bp 1.0 64.3
ndhI-ndhA_p4 TTGACCAATGTACCTTGCCG ACAACTTCTTCCCAACTCCT 50.0 45.0 58.5 56.3 213-214 bp 1.0 64.3
ndhI-ndhA_p5 CGCGTATTGTTTGTTGACCA ACAACTTCTTCCCAACTCCT 45.0 45.0 57.3 56.3 193-194 bp 1.0 62.9