Hotspot

ndhF-rpl32

SSC · 111666-112510 · 845 bp

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Pi 0.0203
MarkerSeek score 68.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
845
variable_sites
17
pi
0.0203
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGGAAGTGGAAGGAAGGGTA TTTAACGCCGCCCAATATCC 50.0 50.0 57.6 58.7 985-1151 bp 1.0 72.9
ndhF-rpl32_p2 TGGAAGTGGAAGGAAGGGTA GCCGCCCAATATCCCTTTTT 50.0 50.0 57.6 58.9 979-1145 bp 1.0 71.4
ndhF-rpl32_p3 TTTTGTTGCCGTCGGAAAAA TTTAACGCCGCCCAATATCC 40.0 50.0 57.4 58.7 1036-1202 bp 1.0 71.0
ndhF-rpl32_p4 TTTTTGTTGCCGTCGGAAAA TTTAACGCCGCCCAATATCC 40.0 50.0 57.4 58.7 1037-1203 bp 1.0 71.0
ndhF-rpl32_p5 TGGAAGTGGAAGGAAGGGTA CTTTTAACGCCGCCCAATATC 50.0 47.6 57.6 58.0 987-1153 bp 1.0 70.3