Hotspot

rrn23-rrn4.5

IRb · 106146-106206 · 61 bp

Back to Blitum

Pi 0.0656
MarkerSeek score 73.80
Primer available yes
Label rrn23-rrn4.5

Feature Metadata

label_name
rrn23-rrn4.5
feature_type
igs
region
IRb
length_bp
61
variable_sites
4
pi
0.0656
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn23-rrn4.5_p1 AAGTAGTAAGCCCACCCCAA TCACTGCACTTCCACTTGAC 50.0 50.0 58.2 58.0 157-160 bp 1.0 79.6
rrn23-rrn4.5_p2 AAGTAGTAAGCCCACCCCAA ATGATAAACGGCTCGTCTCG 50.0 50.0 58.2 57.9 127-130 bp 1.0 79.5
rrn23-rrn4.5_p3 TAGTAAGCCCACCCCAAGAT TCACTGCACTTCCACTTGAC 50.0 50.0 57.7 58.0 154-157 bp 1.0 79.4
rrn23-rrn4.5_p4 GTACCAGTTATCGTGCCCAC TCACTGCACTTCCACTTGAC 55.0 50.0 58.4 58.0 227-230 bp 1.0 78.2
rrn23-rrn4.5_p5 GTGTACCAGTTATCGTGCCC TCACTGCACTTCCACTTGAC 55.0 50.0 58.4 58.0 229-232 bp 1.0 78.2