Hotspot

trnP-UGG-psaJ

LSC · 66604-67033 · 430 bp

Back to Blitum

Pi 0.0211
MarkerSeek score 68.20
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
430
variable_sites
9
pi
0.0211
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 ACCCGTGACATTTTGTACCC GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 516-520 bp 1.0 80.9
trnP-UGG-psaJ_p2 CCCGTGACATTTTGTACCCA GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 515-519 bp 1.0 80.9
trnP-UGG-psaJ_p3 ACAGGATTTGAACCCGTGAC GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 527-531 bp 1.0 80.8
trnP-UGG-psaJ_p4 CAGGATTTGAACCCGTGACA GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 526-530 bp 1.0 80.8
trnP-UGG-psaJ_p5 TGACAGGATTTGAACCCGTG GGTGCCACGGAAAGATATGT 50.0 50.0 58.1 58.0 529-533 bp 1.0 80.8