Hotspot

ycf4-cemA

LSC · 60398-61063 · 666 bp

Back to Blitum

Pi 0.0153
MarkerSeek score 69.60
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
666
variable_sites
10
pi
0.0153
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 CAGAGGTCAAGGAGCCATTC CCACCAAGTAGTAACCCAAGA 55.0 47.6 58.0 56.9 887-901 bp 1.0 65.4
ycf4-cemA_p2 CAGAGGTCAAGGAGCCATTC TCCACCAAGTAGTAACCCAAG 55.0 47.6 58.0 56.9 888-902 bp 1.0 65.4
ycf4-cemA_p3 TTGGCCTATTTCTTGCGTGT CCACCAAGTAGTAACCCAAGA 45.0 47.6 58.1 56.9 808-822 bp 1.0 65.1
ycf4-cemA_p4 TTGGCCTATTTCTTGCGTGT TCCACCAAGTAGTAACCCAAG 45.0 47.6 58.1 56.9 809-823 bp 1.0 65.1
ycf4-cemA_p5 GAGCCATTCCTTTGACTCGT CCACCAAGTAGTAACCCAAGA 50.0 47.6 57.9 56.9 876-890 bp 1.0 64.9