Hotspot

trnT-UGU-trnL-UAA

LSC · 46332-47099 · 768 bp

Back to Blitum

Pi 0.0321
MarkerSeek score 72.30
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
768
variable_sites
24
pi
0.0321
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG AGCCAATTAAGTCCGTAGCG 55.0 50.0 57.9 58.1 827-841 bp 1.0 82.6
trnT-UGU-trnL-UAA_p2 GGATTCGAACCGATGACCAT AGCCAATTAAGTCCGTAGCG 50.0 50.0 57.8 58.1 859-873 bp 1.0 81.6
trnT-UGU-trnL-UAA_p3 GCGATGCTCTAACCTCTGAG CCGTAGCGTCTACCGATTTC 55.0 55.0 57.9 58.2 815-829 bp 1.0 81.2
trnT-UGU-trnL-UAA_p4 GCGATGCTCTAACCTCTGAG GCCAATTAAGTCCGTAGCGT 55.0 50.0 57.9 58.4 826-840 bp 1.0 80.4
trnT-UGU-trnL-UAA_p5 GGATTCGAACCGATGACCAT CCGTAGCGTCTACCGATTTC 50.0 55.0 57.8 58.2 847-861 bp 1.0 80.2