Hotspot

atpH-atpI

LSC · 12971-13742 · 772 bp

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Pi 0.0183
MarkerSeek score 70.30
Primer available yes
Label atpH-atpI

Feature Metadata

label_name
atpH-atpI
feature_type
igs
region
LSC
length_bp
772
variable_sites
14
pi
0.0183
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpH-atpI_p1 ATAACGGAAGCGGCAGAAAT GCGAATCACTGGAAGGTCAT 45.0 50.0 58.0 58.0 823-830 bp 1.0 81.6
atpH-atpI_p2 TACCTTGACCAACTCCAGGT GCGAATCACTGGAAGGTCAT 50.0 50.0 57.9 58.0 879-886 bp 1.0 80.9
atpH-atpI_p3 ATAACGGAAGCGGCAGAAAT TTTTGCAACTTTAGCCGCAG 45.0 45.0 58.0 57.9 852-859 bp 1.0 80.7
atpH-atpI_p4 AGCCAATCCAGCAGCAATAA GCGAATCACTGGAAGGTCAT 45.0 50.0 57.9 58.0 839-846 bp 1.0 80.7
atpH-atpI_p5 AATAACGGAAGCGGCAGAAA GCGAATCACTGGAAGGTCAT 45.0 50.0 58.2 58.0 824-831 bp 1.0 80.4