Hotspot

trnR-UCU-atpA

LSC · 9303-9389 · 87 bp

Back to Blitum

Pi 0.0482
MarkerSeek score 69.70
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
87
variable_sites
4
pi
0.0482
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 AGCCTTCCAAGCTAACGATG TCAATGCTGGAATCAGACCG 50.0 50.0 58.0 58.0 808-809 bp 1.0 80.7
trnR-UCU-atpA_p2 AGCCTTCCAAGCTAACGATG GCAGGAGATGTTTCGGCTTA 50.0 50.0 58.0 58.0 887-888 bp 1.0 80.6
trnR-UCU-atpA_p3 CCTAGCCTTCCAAGCTAACG TCAATGCTGGAATCAGACCG 55.0 50.0 58.1 58.0 811-812 bp 1.0 80.5
trnR-UCU-atpA_p4 CCTAGCCTTCCAAGCTAACG GCAGGAGATGTTTCGGCTTA 55.0 50.0 58.1 58.0 890-891 bp 1.0 80.4
trnR-UCU-atpA_p5 AGCCTTCCAAGCTAACGATG CCAATAGTCGAGACCCAAGC 50.0 55.0 58.0 58.1 905-906 bp 1.0 80.4