Hotspot

trnK-UUU-rps16

LSC · 4058-4727 · 670 bp

Back to Blitum

Pi 0.0271
MarkerSeek score 63.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
670
variable_sites
18
pi
0.0271
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AGAATTTCGACTTAATCAAACGA 50.0 30.4 58.2 54.7 734-738 bp 1.0 43.2
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AGAATTTCGACTTAATCAAACGA 50.0 30.4 58.2 54.7 733-737 bp 1.0 43.2
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT TTTCGACTTAATCAAACGAAG 50.0 33.3 58.2 52.6 730-734 bp 1.0 43.2
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TTTCGACTTAATCAAACGAAG 50.0 33.3 58.2 52.6 729-733 bp 1.0 43.2
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT AGAATTTCGACTTAATCAAACGAAG 50.0 32.0 58.2 56.5 733-737 bp 1.0 43.2