Hotspot

ndhF

SSC · 113480-115738 · 2259 bp

Back to Bletilla

Pi 0.0729

MarkerSeek score 82.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2259
variable_sites: 297
pi: 0.0729
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CCTCAGAAACGGAAGAAGGG	CAGAATTGGTGAATCGGAAACA	55.0	40.9	57.9	57.2	2535-2575 bp	0.5	47.8
ndhF_p2	CTCAGAAACGGAAGAAGGGG	CAGAATTGGTGAATCGGAAACA	55.0	40.9	57.9	57.2	2534-2574 bp	0.5	47.8
ndhF_p3	CAAGTTCCACGTTAGCCAGA	CAGAATTGGTGAATCGGAAACA	50.0	40.9	57.8	57.2	2835-2878 bp	0.5	46.2
ndhF_p4	CCTCAGAAACGGAAGAAGGG	TCAGAATTGGTGAATCGGAAAC	55.0	40.9	57.9	57.0	2536-2576 bp	0.5	45.7
ndhF_p5	CTCAGAAACGGAAGAAGGGG	TCAGAATTGGTGAATCGGAAAC	55.0	40.9	57.9	57.0	2535-2575 bp	0.5	45.7