Hotspot

rpl32-trnL-UAG

SSC · 116853-117515 · 663 bp

Back to Bletilla

Pi 0.0837

MarkerSeek score 85.70

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 663
variable_sites: 97
pi: 0.0837
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 85.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TCCACCGGACAGTCAAAAAG	ATCCGCATAAAAGAGCTGCA	50.0	45.0	58.0	58.0	1383-1445 bp	1.0	91.2
rpl32-trnL-UAG_p2	TCCACCGGACAGTCAAAAAG	TCCGCATAAAAGAGCTGCAT	50.0	45.0	58.0	58.0	1382-1444 bp	1.0	91.2
rpl32-trnL-UAG_p3	TCCACCGGACAGTCAAAAAG	GTCCCGAAGAAATCCAACGA	50.0	50.0	58.0	57.9	1116-1178 bp	1.0	91.0
rpl32-trnL-UAG_p4	TCCACCGGACAGTCAAAAAG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	714-762 bp	1.0	90.7
rpl32-trnL-UAG_p5	TCCACCGGACAGTCAAAAAG	AGCAGCGTGTCTACCAATTT	50.0	45.0	58.0	57.8	707-755 bp	1.0	90.2