Hotspot

rps11-rpl36

LSC · 82059-82228 · 170 bp

Back to Bletilla

Pi 0.0559

MarkerSeek score 86.80

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 170
variable_sites: 17
pi: 0.0559
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 86.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	TGTTGAAACTCGCTTGAACA	ATGTCGCTTGATTCGTAGGC	40.0	50.0	56.1	58.4	341-379 bp	1.0	69.2
rps11-rpl36_p2	CGTGCATTCTTACGTAAACCA	ATGTCGCTTGATTCGTAGGC	42.9	50.0	56.9	58.4	273-311 bp	1.0	68.7
rps11-rpl36_p3	TGTTGAAACTCGCTTGAACATG	ATGTCGCTTGATTCGTAGGC	40.9	50.0	58.0	58.4	341-379 bp	1.0	67.8
rps11-rpl36_p4	TTGTTGAAACTCGCTTGAACA	ATGTCGCTTGATTCGTAGGC	38.1	50.0	56.9	58.4	342-380 bp	1.0	67.2
rps11-rpl36_p5	TGTTGAAACTCGCTTGAACA	CGGGGGCGAATTATAGTCAT	40.0	50.0	56.1	57.2	322-360 bp	1.0	67.0