Hotspot

ycf4-cemA

LSC · 61874-62620 · 747 bp

Back to Bletilla

Pi 0.0609
MarkerSeek score 87.80
Primer available yes
Label ycf4-cemA

Feature Metadata

label_name
ycf4-cemA
feature_type
igs
region
LSC
length_bp
747
variable_sites
79
pi
0.0609
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
87.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf4-cemA_p1 CATTCCCTTGACCCGTACTG AAGATATGGGAGGGAAGCCA 55.0 50.0 58.0 57.8 889-920 bp 1.0 89.1
ycf4-cemA_p2 CATTCCCTTGACCCGTACTG AGATATGGGAGGGAAGCCAA 55.0 50.0 58.0 57.8 888-919 bp 1.0 89.1
ycf4-cemA_p3 ATTCCCTTGACCCGTACTGA AGATATGGGAGGGAAGCCAA 50.0 50.0 58.1 57.8 887-918 bp 1.0 88.9
ycf4-cemA_p4 TTCCCTTGACCCGTACTGAT AAGATATGGGAGGGAAGCCA 50.0 50.0 58.1 57.8 887-918 bp 1.0 88.9
ycf4-cemA_p5 TTCCCTTGACCCGTACTGAT AGATATGGGAGGGAAGCCAA 50.0 50.0 58.1 57.8 886-917 bp 1.0 88.9