Hotspot

atpB-rbcL

LSC · 55638-56611 · 974 bp

Back to Bletilla

Pi 0.106

MarkerSeek score 84.20

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 974
variable_sites: 177
pi: 0.106
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TTTTTCCTCCACGGCGATAG	AGAAACGATCTCTCCAACGC	50.0	50.0	58.0	58.0	2152-2305 bp	1.0	89.4
atpB-rbcL_p2	AGCCACTTTGGATTCTGCAA	AGAAACGATCTCTCCAACGC	45.0	50.0	58.0	58.0	2349-2502 bp	1.0	88.8
atpB-rbcL_p3	AGCCACTTTGGATTCTGCAA	AATCAAGTCCACCCCGTAGA	45.0	50.0	58.0	58.1	2289-2442 bp	1.0	88.6
atpB-rbcL_p4	TTTTTCCTCCACGGCGATAG	GTCCACACAGTTGTCCATGT	50.0	50.0	58.0	58.0	1709-1862 bp	0.75	78.2
atpB-rbcL_p5	AGCCACTTTGGATTCTGCAA	GTCCACACAGTTGTCCATGT	45.0	50.0	58.0	58.0	1906-2059 bp	0.75	77.5