Hotspot

petN-psbM

LSC · 29692-30425 · 734 bp

Back to Bletilla

Pi 0.298

MarkerSeek score 80.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 734
variable_sites: 332
pi: 0.298
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGAGGCTCTTAGTGCTGCT	AGCGGGAAAATGAAATGGGA	50.0	45.0	57.8	57.8	1540-1728 bp	1.0	88.9
petN-psbM_p2	TAGAGGCTCTTAGTGCTGCT	CGTTTATTGCTACTGCGCTG	50.0	50.0	57.8	57.9	1228-1259 bp	0.5	67.4
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	CGTTTATTGCTACTGCGCTG	50.0	50.0	58.2	57.9	903-966 bp	0.5	66.0
petN-psbM_p4	CGCTTGTAGTATGGGGAAGG	CGTTTATTGCTACTGCGCTG	55.0	50.0	57.8	57.9	852-915 bp	0.5	65.8
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	AGCGGGAAAATGAAATGGGA	50.0	45.0	58.2	57.8	1215-1264 bp	0.5	64.9