Hotspot

rpoB-trnC-GCA

LSC · 27217-28680 · 1464 bp

Back to Bletilla

Pi 0.0518

MarkerSeek score 81.90

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1464
variable_sites: 134
pi: 0.0518
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCAAATTAAGACCGCGGGTA	AGTATCGACAGACCTAGCCC	50.0	55.0	58.0	58.0	1819-1900 bp	1.0	89.7
rpoB-trnC-GCA_p2	CCAAATTAAGACCGCGGGTA	GTATCGACAGACCTAGCCCT	50.0	55.0	58.0	58.0	1818-1899 bp	1.0	89.7
rpoB-trnC-GCA_p3	CCAAATTAAGACCGCGGGTA	GCCCTACGGGTTCTCAAAAT	50.0	50.0	58.0	57.9	1841-1922 bp	1.0	88.8
rpoB-trnC-GCA_p4	CCCCTACACAGGCAAATTGT	AGTATCGACAGACCTAGCCC	50.0	55.0	58.1	58.0	2258-2339 bp	1.0	88.3
rpoB-trnC-GCA_p5	CCCCTACACAGGCAAATTGT	GTATCGACAGACCTAGCCCT	50.0	55.0	58.1	58.0	2257-2338 bp	1.0	88.3