Hotspot

atpH-atpI

LSC · 14108-14835 · 728 bp

Back to Bletilla

Pi 0.0559

MarkerSeek score 84.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 728
variable_sites: 69
pi: 0.0559
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AGCCAATCCAGCAGCAATAA	GGCTGCGGCTTATATAGGTG	45.0	55.0	57.9	58.3	711-819 bp	1.0	87.8
atpH-atpI_p2	GCCAATCCAGCAGCAATAAC	GGCTGCGGCTTATATAGGTG	50.0	55.0	57.8	58.3	710-818 bp	1.0	87.3
atpH-atpI_p3	TCCAGCAGCAATAACGGAAG	GGCTGCGGCTTATATAGGTG	50.0	55.0	58.3	58.3	705-813 bp	1.0	87.0
atpH-atpI_p4	AATCCAGCAGCAATAACGGA	GGCTGCGGCTTATATAGGTG	45.0	55.0	57.6	58.3	707-815 bp	1.0	85.7
atpH-atpI_p5	ATCCAGCAGCAATAACGGAA	GGCTGCGGCTTATATAGGTG	45.0	55.0	57.6	58.3	706-814 bp	1.0	85.7