Hotspot

psbK-psbI

LSC · 7668-8138 · 471 bp

Back to Bletilla

Pi 0.0725

MarkerSeek score 83.20

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 471
variable_sites: 63
pi: 0.0725
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CGCCCCATTTTGATTTGGTC	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.0	58.0	1326-1379 bp	1.0	90.4
psbK-psbI_p2	CGCCCCATTTTGATTTGGTC	GTGGACTAAAGCGTCGGATT	50.0	50.0	58.0	58.0	1327-1380 bp	1.0	90.4
psbK-psbI_p3	CTGATGCAAACGATCTGGGA	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.0	58.0	1405-1458 bp	1.0	90.0
psbK-psbI_p4	TCTGATGCAAACGATCTGGG	TGGACTAAAGCGTCGGATTG	50.0	50.0	58.0	58.0	1406-1459 bp	1.0	90.0
psbK-psbI_p5	CTGATGCAAACGATCTGGGA	GTGGACTAAAGCGTCGGATT	50.0	50.0	58.0	58.0	1406-1459 bp	1.0	90.0