Hotspot

ycf5

SSC · 114888-115874 · 987 bp

Back to Blennodia

Pi 0.00405

MarkerSeek score 60.10

Primer available yes

Label ycf5

Feature Metadata

label_name: ycf5
feature_type: gene
region: SSC
length_bp: 987
variable_sites: 4
pi: 0.00405
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf5_p1	GCGGCATAAGATCTTATAAAAGAGA	TGGATTCCTTTTTGTTTGTTACTGT	36.0	32.0	57.1	57.8	1137 bp	1.0	40.3
ycf5_p2	TCTAAAATCGGGTAAAACCTAGT	TGGATTCCTTTTTGTTTGTTACTGT	34.8	32.0	55.1	57.8	1070 bp	1.0	40.3
ycf5_p3	AAAATCGGGTAAAACCTAGT	TGGATTCCTTTTTGTTTGTTACTGT	35.0	32.0	52.0	57.8	1067 bp	1.0	40.3
ycf5_p4	GCGGCATAAGATCTTATAAAAGAG	TGGATTCCTTTTTGTTTGTTACTGT	37.5	32.0	55.8	57.8	1137 bp	1.0	40.3
ycf5_p5	TTCTAAAATCGGGTAAAACCTAGT	TGGATTCCTTTTTGTTTGTTACTGT	33.3	32.0	55.8	57.8	1071 bp	1.0	40.3