Hotspot

ycf10/cemA

LSC · 60556-61245 · 690 bp

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Pi 0.00145
MarkerSeek score 46.30
Primer available yes
Label ycf10/cemA

Feature Metadata

label_name
ycf10/cemA
feature_type
gene
region
LSC
length_bp
690
variable_sites
1
pi
0.00145
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf10/cemA_p1 TGGATTCATAGGCTCACTACA AAATTGGATCAGTGAATCATTT 42.9 27.3 55.9 52.3 833 bp 1.0 40.1
ycf10/cemA_p2 TGGATTCATAGGCTCACTACA AATTGGATCAGTGAATCATTTT 42.9 27.3 55.9 52.3 832 bp 1.0 40.1
ycf10/cemA_p3 TGGATTCATAGGCTCACTACA AAATTGGATCAGTGAATCATTTT 42.9 26.1 55.9 53.2 833 bp 1.0 40.1
ycf10/cemA_p4 AGGCTCACTACATTCTATTCGA AAATTGGATCAGTGAATCATTT 40.9 27.3 56.5 52.3 824 bp 1.0 40.1
ycf10/cemA_p5 AGGCTCACTACATTCTATTCGA AATTGGATCAGTGAATCATTTT 40.9 27.3 56.5 52.3 823 bp 1.0 40.1