Hotspot

ycf1

SSC · 124300-129654 · 5355 bp

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Pi 0.00168

MarkerSeek score 53.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5355
variable_sites: 9
pi: 0.00168
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGGCTCATCTCTCCTCTTTCA	GAATCGGCCGGTCAAGTAG	47.6	57.9	57.9	58.0	323 bp	1.0	63.4
ycf1_p2	TGTCTAGGCTCATCTCTCCTC	GAATCGGCCGGTCAAGTAG	52.4	57.9	57.8	58.0	328 bp	1.0	62.5
ycf1_p3	TGTCTAGGCTCATCTCTCCT	GAATCGGCCGGTCAAGTAG	50.0	57.9	56.6	58.0	328 bp	1.0	60.8
ycf1_p4	AGGCTCATCTCTCCTCTTTCA	AATCGGCCGGTCAAGTAGA	47.6	52.6	57.9	58.4	322 bp	1.0	60.3
ycf1_p5	AGGCTCATCTCTCCTCTTTCA	AGAATCGGCCGGTCAAGTA	47.6	52.6	57.9	58.4	324 bp	1.0	60.3