Hotspot

rbcL

LSC · 54863-56302 · 1440 bp

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Pi 0

MarkerSeek score 29.70

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1440
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TGACTTGTAGGGAGGGACTT	AAAAGATTGAGCCGAGTACA	50.0	40.0	57.3	54.7	1522 bp	1.0	47.9
rbcL_p2	TGACTTGTAGGGAGGGACTT	AAAGATTGAGCCGAGTACAA	50.0	40.0	57.3	54.7	1521 bp	1.0	47.9
rbcL_p3	TGACTTGTAGGGAGGGACTT	AAAAAGATTGAGCCGAGTACA	50.0	38.1	57.3	55.5	1523 bp	1.0	46.5
rbcL_p4	TGACTTGTAGGGAGGGACTT	AAAAGATTGAGCCGAGTACAA	50.0	38.1	57.3	55.5	1522 bp	1.0	46.5
rbcL_p5	TCATGACTTGTAGGGAGGGA	AAAGATTGAGCCGAGTACAA	50.0	40.0	57.1	54.7	1524 bp	1.0	46.1