Hotspot

psbH

LSC · 74519-74740 · 222 bp

Back to Blennodia

Pi 0
MarkerSeek score 25.00
Primer available yes
Label psbH

Feature Metadata

label_name
psbH
feature_type
gene
region
LSC
length_bp
222
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
25.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbH_p1 TGGTATACAAAGTCAACAGATCGT GAACTACCAAAGAAGAATAGGCT 37.5 39.1 58.0 56.0 362 bp 1.0 40.0
psbH_p2 TGGTATACAAAGTCAACAGATCGT ACTACCAAAGAAGAATAGGCT 37.5 38.1 58.0 53.9 360 bp 1.0 40.0
psbH_p3 CGGAATGGTATACAAAGTCAACAG GAACTACCAAAGAAGAATAGGCT 41.7 39.1 57.9 56.0 367 bp 1.0 40.0
psbH_p4 CAACGGAATGGTATACAAAGTCA GAACTACCAAAGAAGAATAGGCT 39.1 39.1 56.9 56.0 370 bp 1.0 40.0
psbH_p5 CGGAATGGTATACAAAGTCAACA GAACTACCAAAGAAGAATAGGCT 39.1 39.1 56.9 56.0 367 bp 1.0 40.0