Hotspot

psbG

LSC · 49140-49817 · 678 bp

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Pi 0.00295

MarkerSeek score 60.40

Primer available yes

Label psbG

Feature Metadata

label_name: psbG
feature_type: gene
region: LSC
length_bp: 678
variable_sites: 2
pi: 0.00295
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbG_p1	ACAAAGTGCCCTGCATCTTT	CATGGCGAAAAGGAGCATTG	45.0	50.0	58.3	58.1	890 bp	1.0	77.2
psbG_p2	ACAAAGTGCCCTGCATCTTT	TGGTCTTAGTTCGTTTCCCG	45.0	50.0	58.3	57.6	867 bp	1.0	74.3
psbG_p3	GACAAAGTGCCCTGCATCTT	CATGGCGAAAAGGAGCATTG	50.0	50.0	58.7	58.1	891 bp	1.0	73.5
psbG_p4	CAAAGTGCCCTGCATCTTTT	CATGGCGAAAAGGAGCATTG	45.0	50.0	57.2	58.1	889 bp	1.0	72.9
psbG_p5	ACAAAGTGCCCTGCATCTTT	GGTCTTAGTTCGTTTCCCGA	45.0	50.0	58.3	57.3	866 bp	1.0	72.0