Hotspot

atpF

LSC · 11318-12621 · 1304 bp

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Pi 0.000779

MarkerSeek score 60.70

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1304
variable_sites: 1
pi: 0.000779
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	CGTCGGCTCTAATGGTTACC	AGTTTAGCTAGAAGAGGAGA	55.0	40.0	58.2	52.1	1395-1415 bp	1.0	41.2
atpF_p2	CGTCGGCTCTAATGGTTACC	AGTTTAGCTAGAAGAGGAGAT	55.0	38.1	58.2	52.8	1395-1415 bp	1.0	41.2
atpF_p3	CGTCGGCTCTAATGGTTACC	AGTTTAGCTAGAAGAGGAGATT	55.0	36.4	58.2	53.7	1395-1415 bp	1.0	41.2
atpF_p4	TTTCGTCGGCTCTAATGGTT	AGTTTAGCTAGAAGAGGAGA	45.0	40.0	57.2	52.1	1398-1418 bp	1.0	41.2
atpF_p5	TCGTCGGCTCTAATGGTTAC	AGTTTAGCTAGAAGAGGAGA	50.0	40.0	57.1	52.1	1396-1416 bp	1.0	41.2