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ndhF-rpl32

SSC · 112876-113740 · 865 bp

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Pi 0.00232

MarkerSeek score 60.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 865
variable_sites: 2
pi: 0.00232
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CCACATACGGCGAAGGTTTT	ACGATGTCCAATATCCCTTCC	50.0	47.6	58.8	57.2	1068-1070 bp	1.0	59.4
ndhF-rpl32_p2	CACATACGGCGAAGGTTTTT	ACGATGTCCAATATCCCTTCC	45.0	47.6	57.0	57.2	1067-1069 bp	1.0	58.3
ndhF-rpl32_p3	CCACATACGGCGAAGGTTTT	CGATGTCCAATATCCCTTCCT	50.0	47.6	58.8	56.9	1067-1069 bp	1.0	57.1
ndhF-rpl32_p4	CCACATACGGCGAAGGTTT	ACGATGTCCAATATCCCTTCC	52.6	47.6	58.2	57.2	1068-1070 bp	1.0	56.9
ndhF-rpl32_p5	CCACATACGGCGAAGGTTTT	AACGATGTCCAATATCCCTTCC	50.0	45.5	58.8	57.9	1069-1071 bp	1.0	56.9