Hotspot

rbcL-accD

LSC · 56303-56981 · 679 bp

Back to Blennodia

Pi 0.00736

MarkerSeek score 63.00

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 679
variable_sites: 5
pi: 0.00736
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GAGTCCTGAACTAGCTGCTG	TGCTTTACTTAGCTCGCCTC	55.0	50.0	57.7	57.7	839-878 bp	1.0	75.8
rbcL-accD_p2	GCTTGCAAATGGAGTCCTGA	TGCTTTACTTAGCTCGCCTC	50.0	50.0	58.5	57.7	850-889 bp	1.0	74.4
rbcL-accD_p3	TGAGGCTTGCAAATGGAGTC	TGCTTTACTTAGCTCGCCTC	50.0	50.0	58.5	57.7	854-893 bp	1.0	74.4
rbcL-accD_p4	GCTGCTGCTTGTGAAGTATG	TGCTTTACTTAGCTCGCCTC	50.0	50.0	57.5	57.7	826-865 bp	1.0	73.8
rbcL-accD_p5	GAGTCCTGAACTAGCTGCTG	GCTTTACTTAGCTCGCCTCT	55.0	50.0	57.7	57.4	838-877 bp	1.0	73.4