Hotspot

trnT-psbD

LSC · 31340-32431 · 1092 bp

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Pi 0.0119
MarkerSeek score 65.70
Primer available yes
Label trnT-psbD

Feature Metadata

label_name
trnT-psbD
feature_type
igs
region
LSC
length_bp
1092
variable_sites
13
pi
0.0119
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-psbD_p1 GTGGTAGAGTAACGCCATGG ACCTACAAAAACGAAGCGGT 55.0 45.0 58.1 58.0 1247-1251 bp 1.0 80.0
trnT-psbD_p2 TCAGTGGTAGAGTAACGCCA ACCTACAAAAACGAAGCGGT 50.0 45.0 58.1 58.0 1250-1254 bp 1.0 79.9
trnT-psbD_p3 CCAAGACGGCCCTTTTAACT ACCTACAAAAACGAAGCGGT 50.0 45.0 58.1 58.0 1269-1273 bp 1.0 79.8
trnT-psbD_p4 ACGGCCCTTTTAACTCAGTG ACCTACAAAAACGAAGCGGT 50.0 45.0 58.1 58.0 1264-1268 bp 1.0 79.7
trnT-psbD_p5 GACGGCCCTTTTAACTCAGT ACCTACAAAAACGAAGCGGT 50.0 45.0 57.8 58.0 1265-1269 bp 1.0 79.1