Hotspot

trnE-trnT

LSC · 30459-31265 · 807 bp

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Pi 0.00124

MarkerSeek score 59.50

Primer available yes

Label trnE-trnT

Feature Metadata

label_name: trnE-trnT
feature_type: igs
region: LSC
length_bp: 807
variable_sites: 1
pi: 0.00124
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-trnT_p1	TTTCGTAGTACCCTACCCCC	GAACCGATGACTTACGCCTT	55.0	50.0	57.8	58.0	950 bp	1.0	78.8
trnE-trnT_p2	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	929 bp	1.0	78.3
trnE-trnT_p3	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	926 bp	1.0	78.1
trnE-trnT_p4	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	912 bp	1.0	77.9
trnE-trnT_p5	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	891 bp	1.0	77.8