Hotspot

psbM-trnD

LSC · 28726-29729 · 1004 bp

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Pi 0.00299

MarkerSeek score 60.60

Primer available yes

Label psbM-trnD

Feature Metadata

label_name: psbM-trnD
feature_type: igs
region: LSC
length_bp: 1004
variable_sites: 3
pi: 0.00299
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD_p1	AGGAACGAGAATGAAGAGTGC	TTCAATTGGTCAGAGCACCG	47.6	50.0	58.0	58.5	1124-1126 bp	1.0	68.2
psbM-trnD_p2	ACGAGAATGAAGAGTGCAGT	TTCAATTGGTCAGAGCACCG	45.0	50.0	56.9	58.5	1120-1122 bp	1.0	67.5
psbM-trnD_p3	GGAACGAGAATGAAGAGTGCA	TTCAATTGGTCAGAGCACCG	47.6	50.0	58.3	58.5	1123-1125 bp	1.0	66.0
psbM-trnD_p4	AACGAGAATGAAGAGTGCAGT	TTCAATTGGTCAGAGCACCG	42.9	50.0	57.6	58.5	1121-1123 bp	1.0	65.3
psbM-trnD_p5	GGAACGAGAATGAAGAGTGC	TTCAATTGGTCAGAGCACCG	50.0	50.0	56.5	58.5	1123-1125 bp	1.0	64.8