Hotspot

trnS-trnT

LSC · 7741-8435 · 695 bp

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Pi 0

MarkerSeek score 62.70

Primer available yes

Label trnS-trnT

Feature Metadata

label_name: trnS-trnT
feature_type: igs
region: LSC
length_bp: 695
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-trnT_p1	CCAACGCTTTAGTCCACTCA	AACCGAGAGACCCTTTAACT	50.0	45.0	57.8	55.8	743-798 bp	1.0	65.0
trnS-trnT_p2	AACGCTTTAGTCCACTCAGC	AACCGAGAGACCCTTTAACT	50.0	45.0	58.2	55.8	741-796 bp	1.0	64.7
trnS-trnT_p3	CCAACGCTTTAGTCCACTCA	AAACCGAGAGACCCTTTAACT	50.0	42.9	57.8	56.6	744-799 bp	1.0	63.3
trnS-trnT_p4	GCTTTAGTCCACTCAGCCAT	AACCGAGAGACCCTTTAACT	50.0	45.0	57.6	55.8	738-793 bp	1.0	63.0
trnS-trnT_p5	AACGCTTTAGTCCACTCAGC	AAACCGAGAGACCCTTTAACT	50.0	42.9	58.2	56.6	742-797 bp	1.0	63.0