Hotspot

rpl22

LSC · 85159-85662 · 504 bp

Back to Blastus

Pi 0.0068

MarkerSeek score 50.30

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 504
variable_sites: 13
pi: 0.0068
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	ACCAAGTCTGAAACCGAGTG	CGATAATGAATGATGATTAGTCCTCA	50.0	34.6	57.8	57.0	577-598 bp	1.0	41.9
rpl22_p2	ACCAAGTCTGAAACCGAGTG	TCGATAATGAATGATGATTAGTCCTCA	50.0	33.3	57.8	58.2	578-599 bp	1.0	41.9
rpl22_p3	ACCAAGTCTGAAACCGAGTG	TCGATAATGAATGATGATTAGTCCTC	50.0	34.6	57.8	56.8	578-599 bp	1.0	41.9
rpl22_p4	ACCAAGTCTGAAACCGAGTG	TCGATAATGAATGATGATTAGTCCT	50.0	32.0	57.8	55.8	578-599 bp	1.0	41.9
rpl22_p5	CCAAGTCTGAAACCGAGTGG	CGATAATGAATGATGATTAGTCCTCA	55.0	34.6	58.5	57.0	576-597 bp	1.0	41.9