Hotspot

psaC

SSC · 118908-119153 · 246 bp

Back to Blastus

Pi 0.00102

MarkerSeek score 22.20

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 1
pi: 0.00102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 22.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	ACGTTTCTGTCCTCCCCATA	ATCTTAGCCAACCCAGGAGT	50.0	50.0	58.1	58.0	1015-1019 bp	1.0	81.1
psaC_p2	TTTGACCGCTGTACATTGCT	ATCTTAGCCAACCCAGGAGT	45.0	50.0	58.1	58.0	911-915 bp	1.0	80.8
psaC_p3	ACGTTTCTGTCCTCCCCATA	TTATAGCTATTGCCGCAGCC	50.0	50.0	58.1	58.2	1215-1238 bp	1.0	80.3
psaC_p4	TTTGACCGCTGTACATTGCT	TTATAGCTATTGCCGCAGCC	45.0	50.0	58.1	58.2	1111-1134 bp	1.0	80.1
psaC_p5	CAAAACCCGTTCTCGCAAAA	ATCTTAGCCAACCCAGGAGT	45.0	50.0	57.8	58.0	512 bp	0.125	43.2