Hotspot

rps15-trnN-GUU

SSC · 125179-131322 · 6144 bp

Back to Blastus

Pi 0.00717
MarkerSeek score 47.50
Primer available yes
Label rps15-trnN-GUU

Feature Metadata

label_name
rps15-trnN-GUU
feature_type
igs
region
SSC
length_bp
6144
variable_sites
121
pi
0.00717
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-trnN-GUU_p1 TCAAAAATCCATTTAACCGATCAGT ATCCCCCGTTGAAAATTCGT 32.0 45.0 57.5 57.8 308-321 bp 1.0 47.7
rps15-trnN-GUU_p2 TCAAAAATCCATTTAACCGATCAGT TATCGAACCTGTGCGTTTGT 32.0 45.0 57.5 57.8 813-826 bp 1.0 44.0
rps15-trnN-GUU_p3 CAAAAATCCATTTAACCGATCAGT TATCGAACCTGTGCGTTTGT 33.3 45.0 56.2 57.8 812-825 bp 1.0 44.0
rps15-trnN-GUU_p4 AATCCATTTAACCGATCAGT TATCGAACCTGTGCGTTTGT 35.0 45.0 52.2 57.8 808-821 bp 1.0 44.0
rps15-trnN-GUU_p5 TCAAAAATCCATTTAACCGATCAGT CGAACCTGTGCGTTTGTCTA 32.0 50.0 57.5 58.5 817-823 bp 0.375 16.2