Hotspot

ndhF-rpl32

SSC · 114963-115360 · 398 bp

Back to Blastus

Pi 0.0416
MarkerSeek score 80.30
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
398
variable_sites
42
pi
0.0416
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.97
markerseek_score
80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGGGAAGTGGAATGAGAGGT GCCTTCAATGCTGTAAAATAGGC 50.0 43.5 58.0 58.6 538-588 bp 1.0 60.6
ndhF-rpl32_p2 TGGGAAGTGGAATGAGAGGT CCTTCAATGCTGTAAAATAGGCT 50.0 39.1 58.0 57.2 537-587 bp 1.0 58.9
ndhF-rpl32_p3 TGGGAAGTGGAATGAGAGGT CCTTCAATGCTGTAAAATAGGCTT 50.0 37.5 58.0 57.8 537-587 bp 1.0 56.0
ndhF-rpl32_p4 TGGGAAGTGGAATGAGAGGT CCTTCAATGCTGTAAAATAGGC 50.0 40.9 58.0 55.8 537-587 bp 1.0 55.7
ndhF-rpl32_p5 TGGGAAGTGGAATGAGAGGT GCCTTCAATGCTGTAAAATAGG 50.0 40.9 58.0 55.8 538-588 bp 1.0 55.7