Hotspot

trnL-UAA-trnF-GAA

LSC · 48813-49113 · 301 bp

Back to Blastus

Pi 0.0227

MarkerSeek score 64.10

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 301
variable_sites: 16
pi: 0.0227
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.5	58.0	325-356 bp	1.0	83.9
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	361-392 bp	1.0	83.1
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	GTGCCAGGAACCAGATTTGA	50.0	50.0	57.5	58.1	376-407 bp	1.0	82.7
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	TCAGTCCTCTGCTCTACCAG	50.0	55.0	57.5	57.9	337-368 bp	1.0	82.7
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	TGTGCCAGGAACCAGATTTG	50.0	50.0	57.5	58.4	377-408 bp	1.0	80.4