Hotspot

psbZ-trnG-UCC

LSC · 37095-37406 · 312 bp

Back to Blastus

Pi 0.0377

MarkerSeek score 69.60

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: LSC
length_bp: 312
variable_sites: 23
pi: 0.0377
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	GACGGCTGGTCAAGTAACAA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	58.1	57.8	438-467 bp	1.0	85.4
psbZ-trnG-UCC_p2	CCCGTTGTTTTTGCTTCTCC	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.9	57.8	459-488 bp	1.0	85.0
psbZ-trnG-UCC_p3	ACGGCTGGTCAAGTAACAAA	CGTCTTCTCCTTGGCAAAGA	45.0	50.0	57.7	57.8	437-466 bp	1.0	83.7
psbZ-trnG-UCC_p4	GTTTTTGCTTCTCCTGACGG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.3	57.8	453-482 bp	1.0	80.6
psbZ-trnG-UCC_p5	TTTTTGCTTCTCCTGACGGC	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	59.0	57.8	452-481 bp	1.0	77.8