Hotspot

trnS-GCU-trnG-UCC

LSC · 7906-8679 · 774 bp

Back to Blastus

Pi 0.0158
MarkerSeek score 58.90
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
774
variable_sites
31
pi
0.0158
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA TACCACTAAACTATACCCGC 50.0 45.0 58.0 54.0 771-840 bp 1.0 54.9
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC TACCACTAAACTATACCCGC 50.0 45.0 58.0 54.0 770-839 bp 1.0 54.9
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA TTACCACTAAACTATACCCGC 50.0 42.9 58.0 54.9 772-841 bp 1.0 53.8
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC TTACCACTAAACTATACCCGC 50.0 42.9 58.0 54.9 771-840 bp 1.0 53.8
trnS-GCU-trnG-UCC_p5 CAATCCGACGCTTTAGTCCA TTTACCACTAAACTATACCCGC 50.0 40.9 58.0 55.6 773-842 bp 1.0 52.1