Hotspot

rps16-trnQ-UUG

LSC · 5676-6672 · 997 bp

Back to Blastus

Pi 0.0107
MarkerSeek score 62.10
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
997
variable_sites
34
pi
0.0107
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 938-1085 bp 1.0 79.7
rps16-trnQ-UUG_p2 GCACGTTGCTTTCTACCACA GAGGTTCGAATCCTTCCGTC 50.0 55.0 59.1 58.1 941-1088 bp 1.0 77.5
rps16-trnQ-UUG_p3 CGTTGCTTTCTACCACATCG TTCGGAGGTTCGAATCCTTC 50.0 50.0 57.2 57.3 942-1089 bp 1.0 75.0
rps16-trnQ-UUG_p4 CGTTGCTTTCTACCACATCG TAAGGCAACGGGTTTTGGTC 50.0 50.0 57.2 58.7 968-1115 bp 1.0 74.8
rps16-trnQ-UUG_p5 CACGTTGCTTTCTACCACATC GAGGTTCGAATCCTTCCGTC 47.6 55.0 57.5 58.1 940-1087 bp 1.0 74.4