Hotspot

rpl32-trnL-UAG

SSC · 120070-120879 · 810 bp

Back to Bistorta

Pi 0.0333
MarkerSeek score 73.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
810
variable_sites
67
pi
0.0333
unique_haplotype_count
9
primer_available
yes
alignment_reliability
0.95
markerseek_score
73.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGCGAAATCTCTTTCTACCGG ATTTCGAAAATGCTGTGCCG 47.6 45.0 57.8 58.0 940-990 bp 1.0 80.5
rpl32-trnL-UAG_p2 GCGAAATCTCTTTCTACCGGT ATTTCGAAAATGCTGTGCCG 47.6 45.0 58.1 58.0 969-989 bp 0.666667 65.5
rpl32-trnL-UAG_p3 GCGAAATCTCTTTCTACCGGT TCCTAAGAGCAGCGTGTCTA 47.6 50.0 58.1 57.9 913-933 bp 0.666667 65.1
rpl32-trnL-UAG_p4 GCGAAATCTCTTTCTACCGGT GAAGTAGCAAAGCCTTGGGT 47.6 50.0 58.1 58.1 1421-1448 bp 0.666667 64.1
rpl32-trnL-UAG_p5 GCGAAATCTCTTTCTACCGGT TGGGTAAAAATAGCACCGGG 47.6 50.0 58.1 57.9 1406-1433 bp 0.666667 63.8