Hotspot

rpl16-rps3

LSC · 84290-84418 · 129 bp

Back to Bistorta

Pi 0.0334

MarkerSeek score 63.10

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 129
variable_sites: 11
pi: 0.0334
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	GACTGCTTCCTTGGATCCAG	TGAACTAACCGAACAGGCTG	55.0	50.0	58.0	57.8	476-480 bp	1.0	82.7
rpl16-rps3_p2	GTCAAAGCGAGTTCAGTTGC	TGAACTAACCGAACAGGCTG	50.0	50.0	58.0	57.8	526-530 bp	1.0	82.6
rpl16-rps3_p3	CAAAGCGAGTTCAGTTGCTG	TGAACTAACCGAACAGGCTG	50.0	50.0	58.0	57.8	524-528 bp	1.0	82.6
rpl16-rps3_p4	CGAGTTCAGTTGCTGCAATG	TGAACTAACCGAACAGGCTG	50.0	50.0	58.0	57.8	519-523 bp	1.0	82.5
rpl16-rps3_p5	TCAAAGCGAGTTCAGTTGCT	TGAACTAACCGAACAGGCTG	45.0	50.0	58.0	57.8	525-529 bp	1.0	82.4