Hotspot

psaJ-rpl33

LSC · 68578-69061 · 484 bp

Back to Bistorta

Pi 0.0389

MarkerSeek score 75.70

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 484
variable_sites: 49
pi: 0.0389
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GGTTTTAGGGAATGCTCCGT	ATTCCAATCGACTCGGTGTG	50.0	50.0	57.9	58.0	1002-1025 bp	1.0	86.1
psaJ-rpl33_p2	ATGGTTCGGATCTTTAGCGG	ATTCCAATCGACTCGGTGTG	50.0	50.0	57.8	58.0	704-717 bp	1.0	86.0
psaJ-rpl33_p3	AAAACAAACGCGCTACCAAG	ATTCCAATCGACTCGGTGTG	45.0	50.0	57.9	58.0	1169-1219 bp	1.0	85.7
psaJ-rpl33_p4	CAAAACAAACGCGCTACCAA	ATTCCAATCGACTCGGTGTG	45.0	50.0	58.2	58.0	1170-1220 bp	1.0	85.2
psaJ-rpl33_p5	TTGTACCCAAAACAAACGCG	ATTCCAATCGACTCGGTGTG	45.0	50.0	57.8	58.0	1177-1227 bp	1.0	85.0