Hotspot

psbE-petL

LSC · 66520-67171 · 652 bp

Back to Bistorta

Pi 0.0162

MarkerSeek score 63.00

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 652
variable_sites: 24
pi: 0.0162
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.8	57.1	686-832 bp	1.0	77.8
psbE-petL_p2	GTTCTCCTGTGCTTCCAGAC	GTCGTATCTTGCTCAGACCA	55.0	50.0	58.2	57.1	681-827 bp	1.0	77.5
psbE-petL_p3	ACGAATAACCAACCCGCAAT	GTCGTATCTTGCTCAGACCA	45.0	50.0	58.2	57.1	772-918 bp	1.0	77.4
psbE-petL_p4	CGCAAAAGAACGTTCTCCTG	GTCGTATCTTGCTCAGACCA	50.0	50.0	57.7	57.1	692-838 bp	1.0	76.6
psbE-petL_p5	TCTCCTGTGCTTCCAGACAT	GTCGTATCTTGCTCAGACCA	50.0	50.0	58.4	57.1	679-825 bp	1.0	76.3