Hotspot

ycf4-cemA

LSC · 62185-63093 · 909 bp

Back to Bistorta

Pi 0.0235

MarkerSeek score 62.90

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 909
variable_sites: 58
pi: 0.0235
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGTGCGT	CGTCCAACAGTAAGAGCTCC	45.0	55.0	58.1	58.0	1109-1181 bp	1.0	88.0
ycf4-cemA_p2	AAATCGTCGCATCTTCCTCC	CCTCGTCCAACAGTAAGAGC	50.0	55.0	58.1	58.0	1293-1365 bp	1.0	87.8
ycf4-cemA_p3	AAATCGTCGCATCTTCCTCC	CGTCCAACAGTAAGAGCTCC	50.0	55.0	58.1	58.0	1290-1362 bp	1.0	87.8
ycf4-cemA_p4	TGAATTGGCGATCAGAACGT	CCTCGTCCAACAGTAAGAGC	45.0	55.0	57.9	58.0	1627-1699 bp	1.0	86.9
ycf4-cemA_p5	TGAATTGGCGATCAGAACGT	CGTCCAACAGTAAGAGCTCC	45.0	55.0	57.9	58.0	1624-1696 bp	1.0	86.9