Hotspot

trnT-UGU-trnL-UAA

LSC · 48124-48988 · 865 bp

Back to Bistorta

Pi 0.0395
MarkerSeek score 69.70
Primer available yes
Label trnT-UGU-trnL-UAA

Feature Metadata

label_name
trnT-UGU-trnL-UAA
feature_type
igs
region
LSC
length_bp
865
variable_sites
86
pi
0.0395
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.94
markerseek_score
69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnL-UAA_p1 GCGATGCTCTAACCTCTGAG TGTGGAGGAAGAGACCCAAT 55.0 50.0 57.9 58.0 1612-1720 bp 0.888889 84.0
trnT-UGU-trnL-UAA_p2 GAGTCGAACCGATGACCATC TGTGGAGGAAGAGACCCAAT 55.0 50.0 58.2 58.0 1643-1751 bp 0.888889 83.7
trnT-UGU-trnL-UAA_p3 AACTGAAGCGAACTCCCTTG TGTGGAGGAAGAGACCCAAT 50.0 50.0 58.1 58.0 2113-2253 bp 0.888889 83.4
trnT-UGU-trnL-UAA_p4 TGAAGCGAACTCCCTTGAAG TGTGGAGGAAGAGACCCAAT 50.0 50.0 57.8 58.0 2110-2250 bp 0.888889 83.0
trnT-UGU-trnL-UAA_p5 ACGTATCCGAAGTTCCTCCT TGTGGAGGAAGAGACCCAAT 50.0 50.0 57.9 58.0 2012-2152 bp 0.777778 78.8